Composite

Part:BBa_K1882016:Design

Designed by: Milena Krach   Group: iGEM16_Tuebingen   (2016-10-19)


Apa-Bam-Promotor-ZFNLbind-RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
    Illegal NheI site found at 44
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

x


Source

BBa_J23110; BBa_B0032 Lombardo et al. Nature Biotechnology 25, 1298 - 1306 (2007) "Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery"

References